Взаимодействие клеток бактерий с соединениями серебра и золота: влияние на рост, образование биопленок, механизмы действия, биогенез наночастиц тема диссертации и автореферата по ВАК РФ 03.01.06, кандидат биологических наук Радциг, Марина Александровна

• Радциг, Марина Александровна
• кандидат биологических наук
• 2013

Оглавление диссертации кандидат биологических наук Радциг, Марина Александровна

I. ВВЕДЕНИЕ.

II. ОБЗОР ЛИТЕРАТУРНЫХ ДАННЫХ.

11.1. ВВЕДЕНИЕ.

11.2. АНТИБАКТЕРИАЛЬНЫЕ ЭФФЕКТЫ ИОНОВ СЕРЕБРА.

II. 2.1. Механизмы действия ионов серебра.

II. 2.2. Резистентность к ионам серебра.

11.3. ДЕЙСТВИЕ НАНОЧАСТИЦ СЕРЕБРА.

II. 3.1. Механизмы действия НЧС.

11.4. ИНГИБИРОВАНИЕ НАНОЧАСТИЦАМИ МИКРООРГАНИЗМОВ ПРИ

ОБЛУЧЕНИИ СВЕТОМ.

II. 4.1. Фотокаталитический механизм.

II.4.2. Фототермальный эффект.

11.5. СИНТЕЗ НАНОЧАСТИЦ.

II.5.1. Биосинтез наночастиц с использованием бактерий.

Заключение диссертации по теме «Биотехнология (в том числе бионанотехнологии)», Радциг, Марина Александровна

VI. выводы

1. Определены закономерности ингибирующего действия ионов серебра, золота и НЧС на планктонный рост и образование биопленок Escherichia coli, Pseudomonas aeruginosa и Serratiaproteamaculans. Эти соединения вызывали разрушение образованных биопленок Е. coli и гибель клеток в них в концентрациях значительно более высоких (в 20-30 раз), чем те, которые подавляют рост и формирование биопленок.

2. Мутации в генах, ответственных за репарацию окислительных повреждений ДНК (mutY, mutS, mutM, mutT, nth), увеличивали чувствительность клеток к НЧС и ионам серебра, что предполагает участие указанных генов в репарации повреждений ДНК, вызванных соединениями серебра.

3. Мутации в генах, участвующих в репарации окислительных повреждений ДНК, не оказывали заметного влияния на чувствительность клеток Е. coli к ионам золота. Это свидетельствует о различиях в механизмах действия на клетки ионов золота и соединений серебра. Не обнаружено антибактериального действия наночастиц золота на клетки бактерий в обычных условиях выращивания.

4. Мутации в генах, ответственных за эксцизионную и SOS - репарацию ДНК, не влияли на чувствительность Е. coli к НЧС, ионам серебра и золота; это показывает, что их антибактериальное действие не связано со значительными повреждениями ДНК, которые могут быть восстановлены при участии этих репаративных систем.

5. QS системы LuxI/LuxR типа не участвовали в контроле чувствительности / устойчивости к соединениям серебра и ионам золота у Р. chlororaphis и Serratia. Мутации в генах глобальных регуляторов Е. coli rpoS, crp, Ion не влияли на чувствительность бактерий к соединениям серебра и золота.

6. Мутантные штаммы Е. coli с инактивированными генами транспортных белков поринов OmpF и ОшрС были в 4 - 8 раз более резистентны к НЧС и ионам серебра, чем штамм дикого типа, что свидетельствует о важной роли поринов в антибактериальном действии этих соединений.

7. Показано, что наночастицы золота при действии фемтосекундного лазерного излучения способствуют оптоперфорации клеточной стенки цианобактерий и биопленок E.coli.

8. Получены стабильные наночастицы золота при культивировании на среде с солыо трехвалентного золота цианобактерий (Nostoc, Anabaena) и Azotobacter в условиях азотфиксации.

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