Активные формы кислорода и их источники в программированной гибели клеток растений и цианобактерии Anabaena variabilis тема диссертации и автореферата по ВАК РФ 03.00.25, кандидат биологических наук Шестак, Анна Александровна

• Шестак, Анна Александровна
• кандидат биологических наук
• 2007

Заключение диссертации по теме «Гистология, цитология, клеточная биология», Шестак, Анна Александровна

Выводы

1. По флуоресценции дихлорфлуоресцеина (DCF) зарегистрировано образование Н2О2 в пленках эпидермиса из листьев гороха, кукурузы и клетках цианобактерии A. variabilis.

2. Цианид вызывал апоптозное разрушение ядер замыкающих клеток устьиц в эпидермисе, изолированном из листьев гороха. Действие CN" усиливалось Н2О2. Нитросиний тетразолий, окисляющий Ог", предотвращал разрушение ядер УК, вызванное CN" или CN~ + Н2О2. Катионное производное убихинона 10-(6'-убихинолил)децилтрифенилфосфоний, избирательно аккумулирующееся в митохондриях, предотвращало CIST -индуцированное разрушение ядер устьичных клеток и подавляло НгОг-зависимое образование 2',7'-дихлорфлуоресцеина в эпидермисе из листьев гороха, вызванное менадионом. Маннитол и этанол, ловушки 'ОН, уменьшали CN" -индуцированный апоптоз. Н2О2, -ОН, а также ресурсы Ог", используемые для генерации Н2О2, участвуют в CNr-индуцированном апоптозном распаде ядер устьичных клеток.

3. Хинакрин, ингибитор флавиновых ферментов, подавлял флуоресцентный ответ DCF на добавление Н2О2 или менадиона. Хинакрин не влиял на дыхание и фотосинтетическое выделение 02 насечками листьев гороха. Возможно, NADPH-оксидаза плазматической мембраны функционирует в качестве источника АФК в программированной гибели клеток.

4. Краситель бенгальский розовый (БР), фотосенсибилирующий образование синглетного кислорода ('02) в сочетании с NADH, окисляемым !0? с образованием Ог", на свету вызывал рост выхода флуоресценции DCF, чувствительный к НСТ. Эти результаты позволяют предполагать

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