Молекулярная характеристика герминальной гранулы, участвующей в репрессии повторов Stellate в семенниках Drosophila melanogaster тема диссертации и автореферата по ВАК РФ 03.01.03, кандидат биологических наук Кибанов, Михаил Викторович
- Специальность ВАК РФ03.01.03
- Количество страниц 115
Оглавление диссертации кандидат биологических наук Кибанов, Михаил Викторович
1. ОБЩАЯ ХАРАКТЕРИСТИКА РАБОТЫ.
1.1 Актуальность проблемы.
1.2 цель и задачи исследования.
1.3 Научная новизна результатов исследования.
1.4 Практическая ценность.
1.5 Апробация работы.
Объем диссертации.
2. ОБЗОР ЛИТЕРАТУРЫ.
2.1 рнк-сайленсинг и короткие РНК.
2.1.1 ¿¡РНК и еэгРНК.
2.1.2 т1РНК.
2.1.3. ргРНК.
2.2 Сперматогенез Бкозорниа меыыооавтея.
2.3 герминальные гранулы йКОБОРН1и МЕЫЫОвАЖЕЯ.
2.3.1 Полярные гранулы.
2.3.2 Nuage.
2.4 герминальные гранулы млекопитающих.
2.4.1 Р1 и ргР-Ьоф в гониоцитах млекопитающих.'.
2.4.2 Хроматоидное тельце: открытие и структура.
2.4.2 Подвижность хроматоидного тельца.
2.4.3 Компоненты хроматоиднцго тельца.
2.4.4. Транспорт в хроматоидное тельце.
2.4.5. Хроматоидное тельце и деградация белков в клетке.
2.4.6 Функции хроматоидного тельца.
2.5 метилирование белков терминальных гранул.
2.5.1 Аргининметилтрансферазы.
2.5.2 Метилирование белков системы р1РНК-сайленсинга.
2.5.3 Тийог-домен-содержащие белки.
2.5.4 Функциональное значение метилирования белков подсемейства РШ1.
3. МАТЕРИАЛЫ И МЕТОДЫ.
3.1 линии втзорнна меыыооазтея, использованные в работе.
3.2 иммунофлуоресцентное окрашивание препаратов целых семенников.
3.3 конфокальная микроскопия.
3.4 получение лизатов для электрофореза и вестерн-блот анализа.
3.5 электрофорез в денатурирующих условиях и вестерн-блот анализ.
3.6 антитела.
3.7 выделение тотальной РНК ОЯОЗОРШи для нозерн-блот гибридизации.
3.8 нозерн-блот гибридизация коротких молекул РНК.
4. РЕЗУЛЬТАТЫ.
4.1 герминальные гранулы в сперматоцитах Drosophila.
4.2 временная динамика существования ping-body.
4.3 Архитектура piNG-body.
4.4 Мутационный анализ: разрушение piNG-body и дерепрессия генов Stellate.
4.5 Влияние метилирования остатков аргинина на формирование piNG-body.
5.0БСУЖДЕНИЕ.
6. ВЫВОДЫ.
Рекомендованный список диссертаций по специальности «Молекулярная биология», 03.01.03 шифр ВАК
Изучение роли белка Piwi в процессах самообновления стволовых клеток и репрессии мобильных элементов у Drosophila melanogaster2013 год, кандидат биологических наук Соколова, Олеся Александровна
Особенности механизма подавления экспрессии генов Stellate с помощью РНК-интерференции у Drosophila melanogaster2009 год, кандидат биологических наук Котельников, Роман Николаевич
Изучение экспрессии и взаимодействий белка Stellate в семенниках Drosophila melanogaster2010 год, кандидат биологических наук Егорова, Ксения Сергеевна
Характеристика биогенеза rasiPHK в герминальной ткани Drosophila melanogaster2007 год, кандидат биологических наук Вагин, Василий Владимирович
Роль РНК-интерференции в подавлении транскрипции ретротранспозонов и тандемных повторов у D. melanogaster2005 год, кандидат биологических наук Кленов, Михаил Сергеевич
Заключение диссертации по теме «Молекулярная биология», Кибанов, Михаил Викторович
6. Выводы
1. В сперматоцитах Drosophila melanogaster среди множества мелких околоядерных гранул nuage, обнаружена крупная терминальная гранула (одна на клетку). Гранула обогащена белками, функционирующими в piPHK-пути (Vasa, Aubergine, AG03, Tudor, Spindle-E, Squash, Cutoff) и miPHK-пути сайленсинга (Belle и AGOl), а также содержит белок DCP1 - компонент системы деградации мРНК;
2. Крупная гранула состоит из нескольких периферийных долей, расположенных вокруг центральной доли. Белки Aubergine, Vasa, Tudor, Spindle-E, Squash, Cutoff, AGOl и DCP1 обнаружены в составе внешних долей, тогда как белок AG03 располагается в центре гранулы;
3. Дерепрессия тандемных повторов Stellate в результате нарушения образования piPHK на фоне мутаций в генах vasa, aub и ago3 ассоциирована с исчезновением крупных гранул в первичных сперматоцитах;
4. В отсутствие симметричного метилирования остатков аргинина в белке подсемейства PIWI Aubergine при мутации в гене аргининметилтрансферазы capsuleen наблюдается дерепрессия повторов Stellate, нарушение образования piPHK Su(Ste) и исчезновение крупных терминальных гранул.
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