Протон-транслоцирующая активность митохондриального Комплекса I тема диссертации и автореферата по ВАК РФ 03.00.04, кандидат биологических наук Галкин, Александр Сергеевич

• Галкин, Александр Сергеевич
• кандидат биологических наук
• 2000

Оглавление диссертации кандидат биологических наук Галкин, Александр Сергеевич

ОГЛАВЛЕНИЕ.

СПИСОК СОКРАЩЕНИЙ.

ВВЕДЕНИЕ.

ОБЗОР ЛИТЕРАТУРЫ.

Комплекс I и организация дыхательной цепи.

Субъединичный состав и фрагменты Комплекса 1.

Полипептидный состав и редокс-компоненты Комплекса I.

Субъединицы, принадлежащие митохондриальному геному.

Субъединицы Комплекса I, принадлежащие ядерному геному.

Нуклеотид-связывающие субъединицы.

Флавинмононуклеотид-связывающие субъединицы.

Железо-серные кластеры.

Прочносвязанные семихиноны как интермедиаты переноса электронов.

Субъединицы ответственные за связывание хинона.

Ацилпереносящий белок.

Структурная модель организации Комплекса 1.

Реакции, катализируемые Комплексом 1.

Прямой перенос электронов.

КАЕ)Н-оксидазная реакция.

ЫА1)Н:хинон-редуктазная реакция.

Окисление ИАОН искусственными акцепторами электронов.

КАОН: фумарат-редуктазная реакция.

Трансгидрогеназная, а также другие МАХ)Р(Н)-зависимые реакции.

Обратный перенос электронов.

Гистерезисное поведение Комплекса 1.

Ингибиторы Комплекса 1.

Ингибиторы ЫАОН связывающего центра.

Дициклогексилкарбодиимид.

Ингибиторы хинон-связывающего центра.

Транслокация протонов ферментами дыхательной цепи и определение стехиометрического коэффициента п (Й+¡2ё) для КАБНгубихинонредуктазной реакции.

Модели протон-транслоцирукяцего механизма Комплекса 1.

МЕТОДЫ ИССЛЕДОВАНИЯ.

Препаративные методы.

Выделение митохондрий сердца быка.

Выделение СМЧ.

Получение деактивированного и активированного препарата

Комплекса I.

Выделение митохондрий сердца крысы.

Аналитические методы.

Регистрация переноса протонов и окисления МАЕ)Н СМЧ.

Регистрация переноса протонов с помощью рН-метра.

Определение концентрации белка.

РЕЗУЛЬТАТЫ ИССЛЕДОВАНИЯ.

Транслокация протонов Комплексом 1 в составе митохондрий.

Транслокация протонов Комплексом I в составе СМЧ.

Определение коэффициента Н+¡2ё для МАБН:убихинон-редуктазной реакции.

Влияние условий измерения на протон-транслоцирующую активность

Комплекса 1.

Измерение при различных концентрациях добавленного ЫАХ)Н.

Влияние количества СМЧ на регистрацию их протонтранслоцирующей активности.

Влияние разобщителей на величину коэффициента Й+¡2ё.

Влияние pH на величину стехиометрического коэффициента Н+¡2е для КАБН'.хинон-редуктазной реакции.

Влияние ротенона на транслокацию протонов.

Транслокация протонов деактивированной формой Комплекса 1.

ОБСУЖДЕНИЕ РЕЗУЛЬТАТОВ.

ВЫВОДЫ.

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