Фолдинг, сборка и стабильность фибритина бактериофага Т4 тема диссертации и автореферата по ВАК РФ 03.00.04, кандидат биологических наук Лондер, Юрий Яковлевич

• Лондер, Юрий Яковлевич
• кандидат биологических наук
• 1999

Оглавление диссертации кандидат биологических наук Лондер, Юрий Яковлевич

Благодарности.

Список сокращений.

I. Введение.

Заключение диссертации по теме «Биохимия», Лондер, Юрий Яковлевич

VI. выводы

1. Устойчивость к додецилсульфату натрия, детектируемая по изменению электрофо-ретической подвижности в полиакриламидном геле с ДСН, может быть использована в качестве характеристического свойства правильно собранных фибритинов.

2. Показано свойство фибритина и его мутантов, содержащих интактный С-концевой домен, восстанавливать тримерность в присутствии 2% додецилсульфата натрия при температуре -20°С.

3. Фибритин содержит 3 домена, устойчивых к додецилсульфату натрия - один соответствует N-концевой части coiled coil; второй - С-концевой части coiled coil; и третий - С-концевому ß-структурному домену. Тримеризация в присутствии ДСН происходит за счет С-концевого домена (фолдона).

4. Петля L11 (ближайшая к С-концевому домену) несущественна для фолдинга, но стабилизирует структуру собранного белка.

5. Сконструирован мутант фибритин В1, для которого получены кристаллы и решается пространственная структура методом рентгеноструктурного анализа.

6. Получены мутанты фибритина, способные к правильной сборке, будучи секрети-рованными в периплазму Е. coli. Этот результат является еще одним аргументом в пользу гипотезы о том, что сборка фибритина в тример протекает пост-трансляционно.

7. Сконструирован пермутантный фибритин, имеющий фолдон на N-конце. Такой белок демонстрирует свойства tsf (temperature-sensitive folding)-мутанта и при пермиссивных условиях (27°С) собирается в стабильную компактную структуру, представляющую собой тримерный coiled coil.

8. По крайней мере, при ренатурации in vitro первым этапом является взаимодействие С-концевых участков, затем происходит формирование coiled coil структуры. С-концевая последовательность является обязательным условием формирования тримеров.

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