Функции регуляторных цистеин-богатых белков вирусов растений тема диссертации и автореферата по ВАК РФ 03.00.06, кандидат биологических наук Луховицкая, Нина Иосифовна

• Луховицкая, Нина Иосифовна
• кандидат биологических наук
• 2006

Оглавление диссертации кандидат биологических наук Луховицкая, Нина Иосифовна

СПИСОК СОКРАЩЕНИЙ

ВВЕДЕНИЕ

ОБЗОР ЛИТЕРАТУРЫ

Gene-for-gene resistance

СI р) кг) pa R-белков

Механизм вшимодействия R-Avr

Индукция сигнальных путей, вызванная взаимодействием R-Avr

Приобретенная сиаемная устойчивость (SAR)

1Ч-!ависимая усюйчивость табака к TMV

Rx-зависиман усюйчивость картофеля к PVX

Тт2.2-твисимая устойчивость томата к ToMV

SwS-завнсимая устойчивость томата к TWSV

HRT-зависимая усюйчивоаь арабидопсиса kTCV

RCY1 зависимая усюйчивос1ь арабидопсиса к CMV-Y

Рецессивные гены ус i ойчивосз и

Р11 (PAMP triggered immunity)

Поспранскрипционноеумолкание генов

Механизм индукции PTGS вирусами растений

РНК-содержащие вирусы

Д11К-содержащие вирусы

Стадии PTGS

Распространение PTGS

С) прессоры PTGS

P19TBSV

He-Pro PVY

2b CMV и ГАУ

25К PVX

CP TCV

TrAP TYLCV

NSsTSWVhNS3RHBV

P15PCV yb BSMV и PSLV

ORF6 TMV

Малые цис геип-бога i ые белки (ЦББ) вирусов рас i ений

МАТЕРИАЛЫ И МЕТОДЫ

РЕЗУЛЬТАТЫ

Влияние ЦББ па развшие инфекции PVX

ЦББ, как возможные факторы авирулентности

ЦББ, как возможные супрессоры «сильного» пути индукции PTGS

ЦББ, как вошожные супрессоры «слабого» пут индукции PTGS

8К, как возможный компонент системы супрессии PTGS PMTV

Дс1скция белка 8К в зараженных растениях

Вн) I риклеточная локализация белка GFP-8K

Вн> I риклеточная локализация белка 12К слито! о с GFP 102 Влияние N-гена на инфекцию PVX-8K, PVX-12K и PVX-15K в N. 104 tabacum

Сравнительный анализ профиля экспрессии ichob N. benthamiana при 115 инфекции PVX дикого тина и рекомбинанзного вируса PVX-12K

Идентификация дифференциально экспрессирукицихся мРНК 115 Свойсша белков, кодируемых дифференциально экспрессируемыми 118 ■енами

Изучение свойств белка 12К CVB

MyraieHi белка 12К CVB.

Влияние 12К и его мутантных вариантов на инфекцию PVX in trans

Способное ib белка 12К связывать РНК и ДНК in vitro

ОБСУЖДЕНИЕ РЕЗУЛЬТАТОВ

ВЫВОДЫ

Введение диссертации (часть автореферата) на тему «Функции регуляторных цистеин-богатых белков вирусов растений»

Настоящая работа посвящена изучению взаимодействия вирусных патогенов ирастений-хо?яев на молекулярном уровне. Исследование молекулярных механизмов,1ежан|их в основе вирусною патогенеза, напрямую связно с изучениемф>ндамен1а.1ьных биочогических процессов в растениях, гаких как передача сигнала,ре1уляция -жсирессии генов, межклеточная коммуникация и программируемаяклеточная смерть В мредставле1пюй работе описана и исследована новая группакодируемых фиювирусами белков, определяющих xapaKiep взаимодействия срастениями хозяевами.Малые неструктурные цистеин-богатые белки (ЦББ) кодируются геномом ряда(+)РНК-содержан1их вирусов растений, К ним относятся вирусы родов Hordeivirus,Tohiavints, Benyvirus, Peduvirm, Allexivirus, Furovirus, Pomovirus и Carlavirus Посгепени структурною сходства ЦББ можно разделить на две группы К первой01 носятся белки, кодируемые вирусами родов Hordeivirus, Tobrcmrus, Benyvirus,Peduviriis и Furovirus И$вестно, что белки этой фуппы участвуют в регуляциирепликации и экспрессии генома вируса, системного транспорта вируса и являютсядетерминантами пато1енности Для ряда ЦББ этой группы была продемопстрированаспособность подавпять PTGS (Dunoyer et al., 2002, Yelina et a l , 2002, Reavy et al., 2004,le et al, 2005) Вторую группу составляют ЦББ, кодируемые вирусами, относяншмисяк двум родам семейсгва Flexiviridae, Allexivirus и Carlavirus. ЦББ PMIV {Pomovirus)неп.зя отнести ни к одной из этих грунн из-за отсутствия сташстически значимогосходства носледовательностеи между ним и дру1 ими.Цель данной работы состояла в изучении функций ЦББ 8К PMTV {Pomovirus), 12КCVB (Сшlavirus) и 15К ShVX {Allexivirus). В ходе работы решались следующиеосновные задачи' Изучение влияния экспрессии ЦББ в составе генома PVX на развитиевирусной инфекции и накопление вирус-сцеццфических РНК в растениях N.benthamiana и N tabacuni; изучение молекулярньЕХ механизмов влияния ЦББ наицд)кцию защитной реакции растении; и-^учение внутриклеточной локализации ЦББ8К PMTV и 12К CVB, картирование районов ЦББ 12К CVB, необходимых для егоф>нкционирования в качестве детерминанты патогенности, изучение снособности 12КCVB связывать нуклеиновые кислоты т vitro

Заключение диссертации по теме «Вирусология», Луховицкая, Нина Иосифовна

выводы

1 Показано, что при экспрессии в контексте PVX ЦББ 8К PMTV, 12К CVB и 15К ShVX увеличивают иаюгснность PVX в растениях N. benthamiana, не являясь при этом супрессорами PTGS или факторами авирулентности.

2 На основании анализа инфекции рекомбинантных вирусов PVX-8K, PVX-12K и PVX-15К в N tabacum cv Samsun показано, что защитный ответ растения на инфекцию рекомбинантными вирусами выражен существенно сильнее в растениях генотипа пп по сравнению с растениями генотипа NN.

3 При сравнительном анализе профиля экспрессии 1енов N. benthamiana в тканях, инфицированных PVX и рекомбинантным вирусом PVX-12K, идентифицированы десять генов, дифференциально экспрессирующихся при инфекции PVX-12K, и выявлено их сходство с генами, ассоциированными с защитной реакцией растения на атаку авирулентных патогенов.

4 Установлено, что кластер положительно заряженных аминокислотных остатков и предполагаемый мотив цинкового пальца в составе 12К CVB необходимы для функционирования этого белка в качестве детерминанты патогенности; показано, что 12К CVB об 1адает PIIK- и ДНК-связывающей активностью in vitro

5 Изучена внутриклеточная локализация ЦББ 8К PMTV и 12К CVB. Показано, что слитый с GFP 8К РМIV локализован в цитонлазматических мембранных структурах, которые являются производными эндоплазматического регикулума, тогда как слитый с GFP 12К CVB локализован в ядре.

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